Nanovaccine Confers Dual Protection Against Influenza A Virus And Porcine Circovirus Type 2
Identifieur interne : 000064 ( Main/Exploration ); précédent : 000063; suivant : 000065Nanovaccine Confers Dual Protection Against Influenza A Virus And Porcine Circovirus Type 2
Auteurs : Peiyang Ding [République populaire de Chine] ; Qianyue Jin [République populaire de Chine] ; Xinxin Chen [République populaire de Chine] ; Suzhen Yang [République populaire de Chine] ; Junqing Guo [République populaire de Chine] ; Guangxu Xing [République populaire de Chine] ; Ruiguang Deng [République populaire de Chine] ; Aiping Wang [République populaire de Chine] ; Gaiping Zhang [République populaire de Chine]Source :
- International Journal of Nanomedicine [ 1176-9114 ] ; 2019.
Abstract
The influenza A virus (IAV) is known for its high variability and poses a huge threat to the health of humans and animals. Pigs play a central role in the cross-species reassortment of IAV. Ectodomain of matrix protein 2 (M2e) is the most conserved protective antigen in IAV and can be used to develop nanovaccines through nanoparticles displaying to increase its immunogenicity. However, the high immunogenicity of nanoparticles can cause the risk of off-target immune response, and excess unwanted antibodies may interfere with the protective efficacy of M2e-specific antibodies. Therefore, it is necessary to select reasonable nanoparticles to make full use of antibodies against nanoparticles while increasing the level of M2e-specific antibodies. Porcine circovirus type 2 (PCV2) is the most susceptible virus in pigs and can promote IAV infection. It is meaningful to develop a vaccine that can simultaneously control swine influenza virus (SIV) and PCV2.
In the present study, M2e of different copy numbers were inserted into the capsid (Cap) protein of PCV2 and expressed in
Cap is capable of carrying at least 81 amino acid residues (three copies of M2e) at its C-terminal without impairing VLPs formation. Cap-3M2e VLPs induced the highest levels of M2e-specific immune responses, conferring protection against lethal challenge of IAVs from different species and induced specific immune responses consistent with PCV2 commercial vaccines in mice. In addition, Cap-3M2e VLPs induced high levels of M2e-specific antibodies and PCV2-specific neutralizing antibodies in pigs.
Cap-3M2e VLP is an economical and promising bivalent nanovaccine, which provides dual protection against IAV and PCV2.
Url:
DOI: 10.2147/IJN.S218057
PubMed: 31571862
PubMed Central: 6754344
Affiliations:
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Ding</name><affiliation wicri:level="1"><nlm:aff id="AFF0001"><institution>Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences</institution>,<addr-line>Zhengzhou</addr-line><addr-line>450002</addr-line>,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0002"><institution>College of Animal Science and Veterinary Medicine, Henan Agricultural University</institution>,<addr-line>Zhengzhou</addr-line><addr-line>450002</addr-line>,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Jin, Qianyue" sort="Jin, Qianyue" uniqKey="Jin Q" first="Qianyue" last="Jin">Qianyue Jin</name><affiliation wicri:level="1"><nlm:aff id="AFF0001"><institution>Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences</institution>,<addr-line>Zhengzhou</addr-line><addr-line>450002</addr-line>,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0003"><institution>School of Life Sciences, Zhengzhou University</institution>,<addr-line>Zhengzhou</addr-line><addr-line>450001</addr-line>,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Chen, Xinxin" sort="Chen, Xinxin" uniqKey="Chen X" first="Xinxin" last="Chen">Xinxin Chen</name><affiliation wicri:level="1"><nlm:aff id="AFF0001"><institution>Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences</institution>,<addr-line>Zhengzhou</addr-line><addr-line>450002</addr-line>,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Yang, Suzhen" sort="Yang, Suzhen" uniqKey="Yang S" first="Suzhen" last="Yang">Suzhen Yang</name><affiliation wicri:level="1"><nlm:aff id="AFF0001"><institution>Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences</institution>,<addr-line>Zhengzhou</addr-line><addr-line>450002</addr-line>,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Guo, Junqing" sort="Guo, Junqing" uniqKey="Guo J" first="Junqing" last="Guo">Junqing Guo</name><affiliation wicri:level="1"><nlm:aff id="AFF0001"><institution>Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences</institution>,<addr-line>Zhengzhou</addr-line><addr-line>450002</addr-line>,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Xing, Guangxu" sort="Xing, Guangxu" uniqKey="Xing G" first="Guangxu" last="Xing">Guangxu Xing</name><affiliation wicri:level="1"><nlm:aff id="AFF0001"><institution>Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences</institution>,<addr-line>Zhengzhou</addr-line><addr-line>450002</addr-line>,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Deng, Ruiguang" sort="Deng, Ruiguang" uniqKey="Deng R" first="Ruiguang" last="Deng">Ruiguang Deng</name><affiliation wicri:level="1"><nlm:aff id="AFF0001"><institution>Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences</institution>,<addr-line>Zhengzhou</addr-line><addr-line>450002</addr-line>,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Aiping" sort="Wang, Aiping" uniqKey="Wang A" first="Aiping" last="Wang">Aiping Wang</name><affiliation wicri:level="1"><nlm:aff id="AFF0004"><institution>Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University</institution>,<addr-line>Yangzhou</addr-line><addr-line>225009</addr-line>,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Zhang, Gaiping" sort="Zhang, Gaiping" uniqKey="Zhang G" first="Gaiping" last="Zhang">Gaiping Zhang</name><affiliation wicri:level="1"><nlm:aff id="AFF0001"><institution>Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences</institution>,<addr-line>Zhengzhou</addr-line><addr-line>450002</addr-line>,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0002"><institution>College of Animal Science and Veterinary Medicine, Henan Agricultural University</institution>,<addr-line>Zhengzhou</addr-line><addr-line>450002</addr-line>,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0003"><institution>School of Life Sciences, Zhengzhou University</institution>,<addr-line>Zhengzhou</addr-line><addr-line>450001</addr-line>,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="AFF0004"><institution>Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University</institution>,<addr-line>Yangzhou</addr-line><addr-line>225009</addr-line>,<country>People’s Republic of China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">International Journal of Nanomedicine</title><idno type="ISSN">1176-9114</idno><idno type="eISSN">1178-2013</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="S2001"><title>Background</title><p>The influenza A virus (IAV) is known for its high variability and poses a huge threat to the health of humans and animals. Pigs play a central role in the cross-species reassortment of IAV. Ectodomain of matrix protein 2 (M2e) is the most conserved protective antigen in IAV and can be used to develop nanovaccines through nanoparticles displaying to increase its immunogenicity. However, the high immunogenicity of nanoparticles can cause the risk of off-target immune response, and excess unwanted antibodies may interfere with the protective efficacy of M2e-specific antibodies. Therefore, it is necessary to select reasonable nanoparticles to make full use of antibodies against nanoparticles while increasing the level of M2e-specific antibodies. Porcine circovirus type 2 (PCV2) is the most susceptible virus in pigs and can promote IAV infection. It is meaningful to develop a vaccine that can simultaneously control swine influenza virus (SIV) and PCV2.</p></sec><sec id="S2002"><title>Methods</title><p>In the present study, M2e of different copy numbers were inserted into the capsid (Cap) protein of PCV2 and expressed in <italic>Escherichia coli</italic> to form self-assembled chimeric virus-like particles (VLPs) nanovaccine. BALB/c mice and pigs were immunized with these nanovaccines to explore optimal anti-IAV and anti-PCV2 immunity.</p></sec><sec id="S2003"><title>Results</title><p>Cap is capable of carrying at least 81 amino acid residues (three copies of M2e) at its C-terminal without impairing VLPs formation. Cap-3M2e VLPs induced the highest levels of M2e-specific immune responses, conferring protection against lethal challenge of IAVs from different species and induced specific immune responses consistent with PCV2 commercial vaccines in mice. In addition, Cap-3M2e VLPs induced high levels of M2e-specific antibodies and PCV2-specific neutralizing antibodies in pigs.</p></sec><sec id="S2004"><title>Conclusion</title><p>Cap-3M2e VLP is an economical and promising bivalent nanovaccine, which provides dual protection against IAV and PCV2.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Ding, Peiyang" sort="Ding, Peiyang" uniqKey="Ding P" first="Peiyang" last="Ding">Peiyang Ding</name></noRegion><name sortKey="Chen, Xinxin" sort="Chen, Xinxin" uniqKey="Chen X" first="Xinxin" last="Chen">Xinxin Chen</name><name sortKey="Deng, Ruiguang" sort="Deng, Ruiguang" uniqKey="Deng R" first="Ruiguang" last="Deng">Ruiguang Deng</name><name sortKey="Ding, Peiyang" sort="Ding, Peiyang" uniqKey="Ding P" first="Peiyang" last="Ding">Peiyang Ding</name><name sortKey="Guo, Junqing" sort="Guo, Junqing" uniqKey="Guo J" first="Junqing" last="Guo">Junqing Guo</name><name sortKey="Jin, Qianyue" sort="Jin, Qianyue" uniqKey="Jin Q" first="Qianyue" last="Jin">Qianyue Jin</name><name sortKey="Jin, Qianyue" sort="Jin, Qianyue" uniqKey="Jin Q" first="Qianyue" last="Jin">Qianyue Jin</name><name sortKey="Wang, Aiping" sort="Wang, Aiping" uniqKey="Wang A" first="Aiping" last="Wang">Aiping Wang</name><name sortKey="Xing, Guangxu" sort="Xing, Guangxu" uniqKey="Xing G" first="Guangxu" last="Xing">Guangxu Xing</name><name sortKey="Yang, Suzhen" sort="Yang, Suzhen" uniqKey="Yang S" first="Suzhen" last="Yang">Suzhen Yang</name><name sortKey="Zhang, Gaiping" sort="Zhang, Gaiping" uniqKey="Zhang G" first="Gaiping" last="Zhang">Gaiping Zhang</name><name sortKey="Zhang, Gaiping" sort="Zhang, Gaiping" uniqKey="Zhang G" first="Gaiping" last="Zhang">Gaiping Zhang</name><name sortKey="Zhang, Gaiping" sort="Zhang, Gaiping" uniqKey="Zhang G" first="Gaiping" last="Zhang">Gaiping Zhang</name><name sortKey="Zhang, Gaiping" sort="Zhang, Gaiping" uniqKey="Zhang G" first="Gaiping" last="Zhang">Gaiping Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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